Medical Laboratories:
Course contents of Inorganic Chemistry
Semester: 1st Number of credits: 1
ID |
Experiment name |
Description |
1 |
General seminar about laboratory |
|
2 |
Introduction of laboratory equipment and laboratory instruction |
|
3 |
Preparation of solution with different percentage, Different normality, Different molarity |
|
4 |
Dilution of different solutions and |
|
5 |
Titration for determination of unknown concentration of acid |
|
6 |
Titration for determination of unknown concentration of base |
|
7 |
Determination of halogens using AgNO3 reagent. |
|
8 |
Identification of CO2 in respiration using Ba(OH)2 reagent. |
|
9 |
Reaction of salt and bases using FeSO4 salt and NaOH base. |
|
10 |
Determination of endothermic and exothermic using NH4Cl and Ba (OH)2 . |
|
11 |
Identification of Nitrites using H2SO4 and Diphenylamine |
|
12 |
Test for Na-acetate to identify the saturated, unsaturated and super saturated solution. |
|
13 |
Test for redox reaction using H2O2 and KMNO4 |
|
14 |
Titration for determination of Zinc Oxide using H2SO4, Phenolphthalein, Ammonium chloride, NaOH, Methyl orange and Potassium tartrate. |
|
15 |
Oxidation of Zinc oxide using heat |
|
16 |
Solving the problems of the students |
Course contents of Cell Biology
Semester: 1st Number of credits: 1
ID |
Experiment name |
Description |
1 |
General seminar about laboratory |
|
2 |
Introduction of laboratory equipment and laboratory instruction |
|
3 |
Introduction of microscope and the method how to use microscope |
|
4 |
Observation of onion cells under microscope |
|
5 |
Observation of oral epithelial cell under microscope |
|
6 |
Epithelial cell staining using methylene blue |
|
7 |
Observation of diffusion using CuSO4 5H2O |
|
8 |
Effect of different solution based on osmotic pressure on RBCs |
|
9 |
Explanation of DNA model 1 |
|
10 |
Explanation of DNA model 2 |
|
11 |
Explanation of cell model |
|
12 |
Explanation of Mitosis model |
|
13 |
Explanation of Meiosis model |
|
14 |
Observation of plasmolysis and hemolysis of RBC |
|
15 |
Study of hair under microscope |
|
16 |
Discussion with the student and solving the student p roblems |
Course contents of Organic chemistry
Semester: 2nd
Number of credits: 1
ID |
Name of experiments |
Description |
1 |
Introduction of laboratory equipment and laboratory instruction |
|
2 |
Ethanol determination using KMnO4 |
|
3 |
Phenol identification using FeCl3 |
|
4 |
Acrolein test for lipids |
|
5 |
Acetic acid identification |
|
6 |
Alkene determination using bromine water |
|
7 |
Identification of formic acid using Na2CO3 and H2SO4 |
|
8 |
Mulish test |
|
9 |
Orthotolidine test |
|
10 |
Preparation of Piranha solution using H2O2 and H2SO4 |
|
11 |
Soap preparation |
|
12 |
Hand sanitizer preparation |
|
13 |
Oxidation of plastic using Piranha Solution |
|
14 |
|
|
15 |
|
|
16 |
Discussion with students and solving the problems of the students |
Course contents of Biochemistry
Semester: 3rd
Number of credits: 1
ID |
Name of experiments |
Description |
1 |
Introduction of laboratory equipment and laboratory instruction |
|
2 |
Benedict test |
|
3 |
Hydrolysis of Sucrose using HCl |
|
4 |
Identification of amylase in saliva and effect of amylase on starch |
|
5 |
Mulish test for determination of carbohydrates |
|
6 |
Seliwanoff’s test for carbohydrate determination |
|
7 |
Barfoed’s test |
|
8 |
Osazone formation of glucose |
|
9 |
Lugol test for identification for starch, glycogen and cellulose |
|
10 |
Strip test for urine to identify the glucose, protein, pH, and density of urine. |
|
11 |
Test for Iodine number in lipids |
|
12 |
Saponification of lipids |
|
13 |
Biuret test for protein identification |
|
14 |
Precipitation of protein using heat, alcohol, and salts. |
|
15 |
Identification of specific gravity of urine using Urinometer |
|
16 |
Discussion with students and solving the problems of the students |
Course contents of Physiology
Semester: 3rd Number of credits: 1
ID |
Name of experiments |
Description |
1 |
Introduction of laboratory equipment and laboratory instruction |
|
2 |
Introduction of blood composition and cell types and demonstration of centrifugation |
|
3 |
Introduction of different blood sampling test tubes |
|
4 |
Phlebotomy methods |
|
5 |
Blood smear formation |
|
6 |
Blood staining using Geimsa and methylene blue |
|
7 |
ESR method by ESR Fast Detector and Westergen method |
|
8 |
Identification of blood hemoglobin using Hemometer and colorimeter. |
|
9 |
Clotting time and bleeding time |
|
10 |
Hematocrit method |
|
11 |
WBC counting using hemocytometer /neubauer chamber |
|
12 |
RBC counting using hemocytometer /neubauer chamber |
|
13 |
Differentiated leucocyte counting ( DLC) using Rapi stain kit |
|
14 |
Differentiated leucocyte counting ( DLC) using Rapi stain kit 1 |
|
15 |
Hemoglobin determination using Sahli’s method |
|
16 |
Hemoglobin determination using colorimeter machine and comparison with Sahli’s method |
|
17 |
Discussion with students and solving the problems of the students |
|
Course contents of Physiology 1
Semester: 3rd
Number of credits: 1
ID |
Name of experiments |
Description |
1 |
Introduction of laboratory equipment and laboratory instruction |
|
2 |
Introduction of blood composition and cell types and demonstration of centrifugation |
|
3 |
Introduction of different blood sampling test tubes |
|
4 |
Phlebotomy methods |
|
5 |
Blood smear formation |
|
6 |
Blood staining using Geimsa and methylene blue |
|
7 |
ESR method by ESR Fast Detector and Westergen method |
|
8 |
Identification of blood hemoglobin using Hemometer and colorimeter. |
|
9 |
Clotting time and bleeding time |
|
10 |
Hematocrit method |
|
11 |
WBC counting using hemocytometer /neubauer chamber |
|
12 |
RBC counting using hemocytometer /neubauer chamber |
|
13 |
Differentiated leucocyte counting ( DLC) using Rapi stain kit |
|
14 |
Differentiated leucocyte counting ( DLC) using Rapi stain kit 1 |
|
15 |
Hemoglobin determination using Sahli’s method |
|
16 |
Hemoglobin determination using colorimeter machine and comparison with Sahli’s method |
|
17 |
Discussion with students and solving the problems of the students |
|
Course contents of Microbiology 1
Semester: 4th
Number of credits: 1
ID |
Name of experiments |
Description |
1 |
Introduction of laboratory equipment and laboratory instruction |
|
2 |
Chemical and physical sterilization |
|
3 |
Sampling method and sample transportation |
|
4 |
Blood agar preparation |
|
5 |
Salmonila and Shegela (SS) agar preparation |
|
6 |
CLED agar preparation |
|
7 |
Bacteria culturing on growth media |
|
8 |
Sub culturing method |
|
9 |
Simple staining |
|
10 |
Negative staining |
|
11 |
Geimsa staining |
|
12 |
Gram staining |
|
13 |
Acid fast staining |
|
14 |
Candida staining methods |
|
15 |
Observation of different type of colony |
|
16 |
Discussion with students and solving the problems of the students |
Course contents of Physiology 2
Semester: 4th Number of credits: 1
ID |
Experiment name |
Description |
1 |
Negatoscope, X-ray screen for X-Ray detection |
|
2 |
Observation of Microcytic and macrocytic anemia
|
|
3 |
HBs, HCV, HIV, H-Pylori, Syphilis Serologic test of |
|
4 |
Com’s test performing of |
|
5 |
Performing of Cross match |
|
6 |
Training for Pulse checking |
|
7 |
Training for blood pressure checking |
|
8 |
Training with statoscope |
|
9 |
Temperature checking with Thermometer |
|
10 |
Vision test |
|
11 |
Tuning fork |
|
12 |
Reflex hummer |
|
13 |
CPR |
|
14 |
Detection of blood pH |
|
15 |
Training for electrocardiogram machine |
|
16 |
Observation of depolarization and repolarization |
|
Course contents of Bacteriology 2
Semester: 5th Number of credits: 1
ID |
Name of experiments |
Description |
1 |
Oxidase test performing |
|
2 |
Performing of catalase test |
|
3 |
Coagulase test using slide method |
|
4 |
Coagulase test using tube method |
|
5 |
Anti-bio gram methods |
|
6 |
MRSA test |
|
7 |
Vidal test |
|
8 |
Immunological tests using strips |
|
9 |
Inoculation techniques in culture media |
|
10 |
Interpretation of cultures in solid media after bacterial growth (Hemolysine) |
|
11 |
H-Pylori test using Ag strip |
|
12 |
Ascaris egg observation |
|
13 |
Protozoa observation under microscope |
|
14 |
Leishmania observation under microscope |
|
15 |
Giardia observation under microscope |
|
16 |
Discussion with students and solving the problems of the students |