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Medical Laboratories

Mili

Medical Laboratories

Course Contents

Inorganic Chemistry

Semester: 1st
Credits: 1

ID	Experiment name	Description
1	General seminar about laboratory
2	Introduction of laboratory equipment and laboratory instruction
3	Preparation of solution with different percentage, Different normality, Different molarity
4	Dilution of different solutions and
5	Titration for determination of unknown concentration of acid
6	Titration for determination of unknown concentration of base
7	Determination of halogens using AgNO3 reagent.
8	Identification of CO2 in respiration using Ba(OH)2 reagent.
9	Reaction of salt and bases using FeSO4 salt and NaOH base.
10	Determination of endothermic and exothermic using NH4Cl and Ba (OH)2 .
11	Identification of Nitrites using H2SO4 and Diphenylamine
12	Test for Na-acetate to identify the saturated, unsaturated and super saturated solution.
13	Test for redox reaction using H2O2 and KMNO4
14	Titration for determination of Zinc Oxide using H2SO4, Phenolphthalein, Ammonium chloride, NaOH, Methyl orange and Potassium tartrate.
15	Oxidation of Zinc oxide using heat
16	Solving the problems of the students

Cell Biology

Semester: 1st
Credits: 1

ID	Experiment name	Description
1	General seminar about laboratory
2	Introduction of laboratory equipment and laboratory instruction
3	Introduction of microscope and the method how to use microscope
4	Observation of onion cells under microscope
5	Observation of oral epithelial cell under microscope
6	Epithelial cell staining using methylene blue
7	Observation of diffusion using CuSO4 5H2O
8	Effect of different solution based on osmotic pressure on RBCs
9	Explanation of DNA model 1
10	Explanation of DNA model 2
11	Explanation of cell model
12	Explanation of Mitosis model
13	Explanation of Meiosis model
14	Observation of plasmolysis and hemolysis of RBC
15	Study of hair under microscope
16	Discussion with the student and solving the student p roblems

Organic Chemistry

Semester: 2nd
Credits: 1

ID	Name of experiments	Description
1	Introduction of laboratory equipment and laboratory instruction
2	Ethanol determination using KMnO4
3	Phenol identification using FeCl3
4	Acrolein test for lipids
5	Acetic acid identification
6	Alkene determination using bromine water
7	Identification of formic acid using Na2CO3 and H2SO4
8	Mulish test
9	Orthotolidine test
10	Preparation of Piranha solution using H2O2 and H2SO4
11	Soap preparation
12	Hand sanitizer preparation
13	Oxidation of plastic using Piranha Solution
14
15
16	Discussion with students and solving the problems of the students

Biochemistry

Semester: 3rd
Credits: 1

ID	Name of experiments	Description
1	Introduction of laboratory equipment and laboratory instruction
2	Benedict test
3	Hydrolysis of Sucrose using HCl
4	Identification of amylase in saliva and effect of amylase on starch
5	Mulish test for determination of carbohydrates
6	Seliwanoff’s test for carbohydrate determination
7	Barfoed’s test
8	Osazone formation of glucose
9	Lugol test for identification for starch, glycogen and cellulose
10	Strip test for urine to identify the glucose, protein, pH, and density of urine.
11	Test for Iodine number in lipids
12	Saponification of lipids
13	Biuret test for protein identification
14	Precipitation of protein using heat, alcohol, and salts.
15	Identification of specific gravity of urine using Urinometer
16	Discussion with students and solving the problems of the students

Physiology

Semester: 3rd
Credits: 1

ID	Name of experiments	Description
1	Introduction of laboratory equipment and laboratory instruction
2	Introduction of blood composition and cell types and demonstration of centrifugation
3	Introduction of different blood sampling test tubes
4	Phlebotomy methods
5	Blood smear formation
6	Blood staining using Geimsa and methylene blue
7	ESR method by ESR Fast Detector and Westergen method
8	Identification of blood hemoglobin using Hemometer and colorimeter.
9	Clotting time and bleeding time
10	Hematocrit method
11	WBC counting using hemocytometer /neubauer chamber
12	RBC counting using hemocytometer /neubauer chamber
13	Differentiated leucocyte counting ( DLC) using Rapi stain kit
14	Differentiated leucocyte counting ( DLC) using Rapi stain kit 1
15	Hemoglobin determination using Sahli’s method
16	Hemoglobin determination using colorimeter machine and comparison with Sahli’s method
17	Discussion with students and solving the problems of the students

Microbiology 1

Semester: 4th
Credits: 1

ID	Name of experiments	Description
1	Introduction of laboratory equipment and laboratory instruction
2	Introduction of blood composition and cell types and demonstration of centrifugation
3	Introduction of different blood sampling test tubes
4	Phlebotomy methods
5	Blood smear formation
6	Blood staining using Geimsa and methylene blue
7	ESR method by ESR Fast Detector and Westergen method
8	Identification of blood hemoglobin using Hemometer and colorimeter.
9	Clotting time and bleeding time
10	Hematocrit method
11	WBC counting using hemocytometer /neubauer chamber
12	RBC counting using hemocytometer /neubauer chamber
13	Differentiated leucocyte counting ( DLC) using Rapi stain kit
14	Differentiated leucocyte counting ( DLC) using Rapi stain kit 1
15	Hemoglobin determination using Sahli’s method
16	Hemoglobin determination using colorimeter machine and comparison with Sahli’s method
17	Discussion with students and solving the problems of the students

Physiology 2

Semester: 4th
Credits: 1

ID	Name of experiments	Description
1	Introduction of laboratory equipment and laboratory instruction
2	Chemical and physical sterilization
3	Sampling method and sample transportation
4	Blood agar preparation
5	Salmonila and Shegela (SS) agar preparation
6	CLED agar preparation
7	Bacteria culturing on growth media
8	Sub culturing method
9	Simple staining
10	Negative staining
11	Geimsa staining
12	Gram staining
13	Acid fast staining
14	Candida staining methods
15	Observation of different type of colony
16	Discussion with students and solving the problems of the students

Physiology 2

Semester: 4th
Credits: 1

ID	Experiment name	Description
1	Negatoscope, X-ray screen for X-Ray detection
2	Observation of Microcytic and macrocytic anemia
3	HBs, HCV, HIV, H-Pylori, Syphilis Serologic test of
4	Com’s test performing of
5	Performing of Cross match
6	Training for Pulse checking
7	Training for blood pressure checking
8	Training with statoscope
9	Temperature checking with Thermometer
10	Vision test
11	Tuning fork
12	Reflex hummer
13	CPR
14	Detection of blood pH
15	Training for electrocardiogram machine
16	Observation of depolarization and repolarization

Physiology 2

Semester: 5th
Credits: 1

ID	Name of experiments	Description
1	Oxidase test performing
2	Performing of catalase test
3	Coagulase test using slide method
4	Coagulase test using tube method
5	Anti-bio gram methods
6	MRSA test
7	Vidal test
8	Immunological tests using strips
9	Inoculation techniques in culture media
10	Interpretation of cultures in solid media after bacterial growth (Hemolysine)
11	H-Pylori test using Ag strip
12	Ascaris egg observation
13	Protozoa observation under microscope
14	Leishmania observation under microscope
15	Giardia observation under microscope
16	Discussion with students and solving the problems of the students