Development of an Infection Model to Study Diabetes Mellitus Type II in Mice Due to Gut Colonization of Staphylococcus aureus
Key Words: Adipose tissues, Insulin, Methicillin resistant staphaphylococcus aureus (MRSA), Obesity, Probiotics
Ajmal Khan Manduzai
Abstract
Diabetes Mellitus Type 2 (DMII) is a long-term chronic illness in which the patient experiences elevated glucose level in their blood as compared to the normal healthy individuals. The various body cells in DMII patients become less sensitive to uptake glucose present in the blood thus this elevated level of glucose in the blood leads individuals to experience life-threatening complications such as cardiovascular, nephropathy, retinopathy and neuropathy. Recent advancement in bioinformatics and molecular tools has made it easy to study the difference between the gut microbial flora of DMII and non-diabetic patients thus reveals that alteration in the composition of gut flora contributes to the onset of insulin resistance and impaired glucose tolerance. Current study revealed that Staphylococcus aureus colonized the gut of the mice and triggered increase in the weight and blood glucose level in mice which are the early important symptoms of DMII. In current experiment, we exposed mice to Staphylococcus aureus through various routes including retro-orbital, oral (food and water) and intra-peritoneal to determine Staphylococcus aureus gut colonization, changes in blood glucose level, weight and food uptake ability. Amongst various routes the intra-peritoneal route of administration resulted in successful Staphylococcus aureus colonization in the gut of experimental mice for six weeks that ultimately resulted in elevation in the blood glucose level, increase in the weight and alteration in the food uptake ability of model organism. Our findings suggest that targeting Staphylococcus aureus in the gut with environment and patient friendly strategies such as probiotics may help scientists to overcome and reduce the spread of DMII in future.
